What color is Alexa Fluor 680?
red
About Alexa Fluor 680 Alexa Fluor® 680 (AF680, Alexa 680) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 695/40 nm bandpass filter.
What does Alexa Fluor 594?
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines….Alexa Fluor 594 protein/antibody labeling kits.
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| 1 mg antibody/protein | Alexa Fluor 594 Antibody/Protein Labeling Kit | A10239 |
What color is DAPI?
blue
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.
What color is Tritc?
bright orange-
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
Who owns Alexa Fluor?
Thermo Fisher Scientific –
Alexa Fluor 488 dye | Thermo Fisher Scientific – US.
What is Alexa Fluor used for?
Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules.
Can DAPI enter live cells?
DAPI is generally used to stain fixed cells since the dye is cell impermeant, although the stain will enter live cells when used at higher concentrations. For live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain.
Why does DAPI bind to DNA?
It is believed that DAPI associates with the minor groove of double-stranded DNA, with a preference for the adenine-thymine clusters. Cells must be permeabilized and/or fixed for DAPI to enter the cell and to bind DNA. Fluorescence increases approximately 20-fold when DAPI is bound to double-stranded DNA.
What does Tritc stain for?
The Invitrogen Alexa Fluor phalloidin conjugates provide researchers with fluorescent probes that are designed to be superior in brightness and photostability to all other spectrally similar conjugates tested.
Why do people prefer Alexa Fluor 488 rather than FITC?
In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments. These properties, i.e. increased sensitivity and environmental stability, make Alexa Flour 488 suitable for intracellular staining.
What does Alexa Fluor bind to?
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
